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KMID : 1134820100390070953
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Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 7 p.953 ~ p.959
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Biological Compounds Extracted from Codium fragile by Enzymatic Hydrolysis and Their Biological Activities
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Lee Ka-Hwa
Senevirathne Mahinda
Ahn Chang-Bum
Je Jae-Young
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Abstract
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We extracted bioactive materials from Codium fragile by enzymatic hydrolysis using four different proteases (Alcalase, Flavourzyme, Neutrase, and Protamex) and seven different carbohydrases (amyloglucosidase (AMG), Celluclast, Dextrozyme, Maltogenase, Promozyme, Termamyl, and Viscozyme), and evaluated their biological activities such as antioxidant, anti-acetylcholinesterase (AChE), and anti-inflammatory effects. All enzymatic hydrolysates showed good DPPH radical scavenging capacities, in particular, Flavourzyme and Promozyme hydrolysates possessed the highest activity. The two hydrolysates also exhibited strong hydrogen peroxide scavenging activity, Fe²? chelating activity, and reducing power in a dose-dependent manner. Furthermore, the two hydrolysates effectively protected DNA damage induced by hydroxyl radical by measuring the conversion of supercoiled DNA to the open circular DNA. All enzymatic hydrolysates also showed high anti-AChE inhibitory activities in a dose-dependent manner, and did not showed any significant cytotoxicity on RAW264.7 cells (p<0.05). In addition, the enzymatic hydrolysates significantly (p<0.05) inhibited lipopolysaccharide inducednitric oxide production on RAW264.7 cells. These results suggest that the enzymatic extracts from Codium fragile would be good source as an ingredient of functional foods.
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KEYWORD
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Codium fragile, antioxidant, anti-acetylcholinesterase, anti-inflammation, enzymatic hydrolysis
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